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. Author manuscript; available in PMC: 2021 Feb 9.
Published in final edited form as: Matrix Biol. 2020 Oct 20;95:52–67. doi: 10.1016/j.matbio.2020.10.004

Fig 7. Collagen XII is associated with the pericellular matrix and influences collagen I secretion.

Fig 7.

Collagen I secretion was analyzed in primary tenocytes obtained from Col12a1+/+ and Col12a1−/− FDLs. Cell layer and culture supernatant were harvested at 1 (A) and 3 (B) days after confluence and analyzed for collagens I and XII content by Western blotting. b-actin is used as an internal control. Collagen XII was found in cell layer at day1 and 3, but not in the supernatant (A, B). Collagen I was found in both the cell layer and culture supernatant as expected. In the cell layer, the collagen I content is comparable between genotypes on both culture days. However, collagen I in the supernatant is decreased in Col12a1−/− cultures at both time points when compared to wild type. (C,D) Quantitative analysis of collagen I at day 1 (C) and day 3 (D) was performed. In wild type controls, more collagen I is present in the supernatant than in the cell layer at both time points, whereas there is less in the Col12a1−/−cell layer. Compared to wild type controls, a significant reduction of collagen I in Col12a1−/− supernatants is found in both culture days.