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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Exp Neurol. 2021 Jan 7;337:113597. doi: 10.1016/j.expneurol.2021.113597

Fig. 1.

Fig. 1.

Ex vivo uncoupling of isolated mitochondria with DNP exert age-dependent effects. Mitochondria were isolated from 4- and 14-MO mice at 3- and 7-days post sham (laminectomy) or spinal cord injury (SCI). Graded doses of DNP were administered to determine the efficiency of generating an uncoupling response. At 3- days post-injury (DPI) maximum respiratory capacity was lower in mitochondria obtained from SCI− compared to sham-mice (A), no injury effect was detectable by 7-DPI (B). All groups exhibited a significant uncoupling response upon administration of 10 μM DNP at 3-DPI (p < 0.005) except mitochondria obtained from 4-MO SCI-mice (C). This age-dependent response was ameliorated by 7-DPI where all groups exhibited a significant uncoupling response upon administration of 10 μM DNP (p < 0.005; D). Analyzed using two-way ANOVA. Graphs represent mean ± SEM, n = 3/group. *p < 0.05, ***p < 0.005 vs vehicle (A, B) or vs baseline (C, D).