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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Exp Neurol. 2021 Jan 7;337:113597. doi: 10.1016/j.expneurol.2021.113597

Fig. 2.

Fig. 2.

The biomarker of reactive oxygen species production, 3-NT, is reduced in 14-MO mice treated with DNP at 7 days after spinal cord injury. DNP was provided at 1.0-, 2.5-, or 5.0-mg/kg/day for 7 days to 4- and 14-MO mice after SCI to determine the effects of uncoupling on accumulation of oxidative stress. Immunohistochemistry was performed to label nitrotyrosine (3-NT) adducts that are indicative of upstream reactive oxygen species production. The 3-NT labeled tissue surrounding the lesion was analyzed as a percentage of the total cross sectional area of the cord. Images of representative samples 500 μm away from the lesion epicenter (A) reveal a significant increase in 3-NT expression in 14-MO mice after SCI (p < 0.05; B). A 1.0 mg/kg/day dose of DNP significantly reduced 3-NT accumulation only in 14-MO mice after SCI (p < 0.05; B). When compared to vehicle-treated mice, no significant differences were found in a treatment effect with DNP between 4- and 14-MO mice (p = 0.09; C). Scale bar represents 500 μm. Analyzed using two-way ANOVA. Graphs represent mean ± SEM, n = 3-5/group. *p < 0.05.