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. 2020 Nov 7;95(2):673–691. doi: 10.1007/s00204-020-02945-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — In vitro cytotoxicity of different EOs. Various human cell lines were seeded into 96-well plates (Hela: 40.000, Caco-2: 120.000, STF1: 40.000 cells/well) and grown over night at 37 °C. Cells were subsequently treated with the indicated EOs and varying concentrations for 24 h. Cell viability was measured using a resazurin-based in vitro toxicology assay. Cell viability of a Hela cells, b Caco-2 cells, and c STF1 cells. d Calculated IC₅₀ values. Error bars are based on the SE of 3 independent measurements