Fig. 4.

Quantitative assessment of DART in the presence of different concentrations of indicated EOs using wild type C. elegans. a Newly hatched nematodes (5 individual L1 animals) were plated onto NGM agar (containing the respective amount of essential oil), seeded with bacterial food in a 24-well plate format and allowed to grow and develop. Wells were imaged on day 6–7, when control animals plated in the absence of EOs had depleted the bacterial food source. Scale bar = 500 µm. b For quantitation of the delay in bacterial food source consumption, wells were scored twice a day for food depletion and the day that control worms depleted the food source was defined as day 0. Error bars are based on the SE of three independent experiments. ** p < 0.01, and **** p < 0.0001 for comparison of delay in bacterial lawn consumption upon EO treatment. ns not significant. c Comparative heatmap for different toxicity parameters as observed in daily DART scoring