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Principle of HET-CAM scoring assay and evaluation of EO irritation potential. Representative CAM photographs illustrating the effect on the membrane over a time period of 5 min of 0.5 ml saline solution (0.9%, negative control) (a), 0.5 ml NaOH solution (0.1 M, positive control) (b), and 10% EO treatment (c–e). Blue and yellow arrow heads indicate haemorrhage and vascular lysis, respectively (as shown in b). f Scoring of acute mucous membrane irritation potential of EOs
