Table 2.

Estimation of oral LD₅₀ based on measured IC₅₀/LC₅₀ values obtained with alternative in vitro and in vivo models

		Rosemary oil			Citrus oil			Eucalyptus oil
			Mean LD50 predicted [g/kg]	LD50 literature1 [g/kg]		Mean LD50 predicted [g/kg]	LD50 literature [g/kg]		Mean LD50 predicted [g/kg]	LD50 literature [g/kg]
Cell culture	Mean IC50 [mg/mL]	0.72	2.8	2.0–5.5	1.2	2.9	2.8–5.1	1.5	3.1	2.5–4.4
	Predicted LD50 [g/kg]	1.2			1.5			1.6
WT nematode	Mean LC50 [mg/mL]	30.6			37.8			36.9
	Predicted LD50 [g/kg]	4.9			5.2			5.2
bus5 nematode	Mean LC50 [mg/mL]	3.6			3.1			4.3
	Predicted LD50 [g/kg]	2.2			2.1			2.4

¹Rat LD₅₀ (oral) values (for all three oils) for comparison were obtained from material safety data sheets of commercially available EOs