Fig. 3.

Steroid release in placental explants over 7 days. Progesterone (a) and estrone (b) release per day in three individual placentas measured in 12 wells. Data points represent mean ± SD (a, b). Effects of the addition of 50 µM forskolin on relative change in progesterone (c) and estrone (d) release over 72 h compared to vehicle control (days 5–7). Relative progesterone or estrone release rate per well per 24 h as compared to baseline conditions (0 h) was calculated and normalized against the corresponding control. Bars represent mean + SEM. Statistical analysis was performed with a two-way repeated measures ANOVA and means were compared to control using a Bonferroni post-hoc test. *P < 0.05, **P < 0.01, ***P < 0.001 (c, d), FK Forskolin