Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 8;12(2):160. doi: 10.1038/s41419-021-03452-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — A Cell viability measured via an MTT assay revealed a significant increase in the death of cultured NRK-52E cells exposed to TGF-β1 (10 ng/ml) in a time-dependent manner. B TGF-β1 caused a marked decrease in mRNA expression of ferroptosis-related molecules Slc7a11 and Gpx4 in NRK-52E cells. C Expression of xCT and GPX4 protein was significantly decreased in TGF-β1-stimulated NRK-52E cells compared to control cells. D Glutathione concentration was significantly decreased in cultured NRK-52E cells exposed TGF-β1 (10 ng/ml) after 6, 12, and 24 h. E TGF-β1 significantly induced lipid peroxidation in NRK-52E cells after 6, 12, and 24 h. F Lipid peroxidation assessed using image-iT^® revealed an increase in TGF-β1-treated NRK-52E cells after 12 h. As shown is representative of three independent replicates. One-way ANOVA and Bonferroni post hoc tests were used for statistical analysis. Error bars represent SD. Original magnification, ×40 for all. Scale bar = 20 µm. *P < 0.05; ***P < 0.001 versus 0 h group.