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. 2020 Sep 30;37(2):145–165. doi: 10.1007/s12264-020-00581-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Plasma membrane distribution of neuronal hASIC1a labeled by the hASIC1a ectodomain antibody. A Representative images of neuronal surface hASIC1a stained by antibody ASC06-IgG1. Surface labeling by ASC06-IgG1 (green) and immunostaining for mCherry (red) in Asic1a^−/− neurons co-transfected with hASIC1a and mCherry. Boxed areas are enlarged to show the location of surface hASIC1a in soma, axon and dendrite. Arrowheads indicate axons; scale bars: left, 50 μm; top right, 10 μm; middle right, 20 μm; bottom right, 5 μm. B, C Relative localization of surface hASIC1a with PSD-95. B A representative dendrite segment and a magnified spine with surface staining by ASC06-IgG1 (red) and immunostaining for PSD-95 (green) and mCherry (magenta) in an Asic1a^−/− neuron co-transfected with mCherry and hASIC1a. Scale bars: upper right, 10 μm; lower right, 1 μm. C Plot for the line profile of ASC06-IgG1 (red) and PSD-95 (green) along the dashed lines in the spine shown in (B). D, E Relative localization of surface hASIC1a with GluN1. D A representative dendrite segment and a magnified spine with surface staining by ASC06-IgG1 (red) and immunostaining for GFP-GluN1 (green) and mCherry (magenta) in an Asic1a^−/− neuron co-transfected with hASIC1a, GFP-GluN1 and mCherry. Scale bars: upper right, 10 μm; lower right, 1 μm. E Plot for the line profile of ASC06-IgG1 (red) and GFP-GluN1 (green) along the dashed line in the spine shown in (D).