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. 2020 Sep 30;37(2):145–165. doi: 10.1007/s12264-020-00581-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Lateral mobility of neuronal surface hASIC1a. A Diagram of direct labeling of surface hASIC1a by Alexa 488-conjugated ASC06-IgG1. B–D SPT experiments on neuronal surface hASIC1a. Asic1a^−/− neurons transfected with hASIC1a were incubated with a low concentration of ASC06-IgG1-Alexa 488. SPT was performed for 1 min–2 min using TIRF microscope at 20 Hz. B Representative image of a neuron labeled by ASC06-IgG1-Alexa 488 under live conditions (left panel) and enlarged reconstructed trajectories of surface hASIC1a clusters in the boxed areas (middle and right panels). Scale bars: left panel, 10 μm; middle and right panels, 1 μm. C, D Quantification of SPT on distribution frequencies of track speeds (C) and track lengths (D) of surface hASIC1a particles (n = 681 particles counted). Track speed = track length/track time. E Mean square displacement (MSD) as a function of time for surface hASIC1a clusters on dendritic shafts and spines. Data points represent the mean ± SEM of 17 cells. Paired t test on area under the curve showed no significant difference between shafts and spines.