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. 2021 Feb 8;12(2):165. doi: 10.1038/s41419-021-03437-w

Fig. 2. The putative cleavage site in MRE11 lies within a 22 amino acid region, namely amino acids 559–580.

Fig. 2

A A map of the lentiviral vector including either intact MRE11 or MRE11 with the initial putative cleavage site (PCS) at amino acids 539–600 removed. The functional domains of MRE11 are indicated50,51. B Schematic representation of MRE11 with the various mutants, including complete deletion of the region (MRE11 PCS removed) and sequential deletions of 12 amino acids (MRE11 Δ539–550, Δ549–560, Δ559–570, Δ569–580, Δ579–590 and Δ589–600). C The MRE11 mutant lacking the initial putative cleavage site failed to generate truncated MRE11. D MRE11 mutants lacking either 559–570 or 569–580 aa did not generate the approximately 20 kDa size short form of cleaved MRE11 in RT112 cells, as seen using anti-myc-tag and anti-MRE11 ab 30725 antibodies. E pLenti-TR-MRE11 vector was designed by deleting 569–708 aa from FL-MRE11 (amino acids deleted shown in upper panel). Lower panel shows the truncation in diagrammatic form.