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. 2021 Feb 8;12(2):165. doi: 10.1038/s41419-021-03437-w

Fig. 5. Cells expressing TR-MRE11 are more radiosensitive and deficiency in HR repair than those expressing FL-MRE11.

Fig. 5

A The protein expression levels of the MRN complex subunits were unchanged after IR (2 Gy). MRE11 KD and TR-MRE11 showed less increased level of γH2AX compared to WT after IR. B GFP based reporter assay showing the efficiency of HR repair pathway under FL-MRE11 or TR-MRE11 rescued condition as indicated. Graph represents the quantifications from three independent experiments (*P < 0.05; one-way ANOVA, n = 3, mean + SEM). C Clonogenic survival rates of TR-MRE11 and KD cells were lower than for FL-MRE11 and untreated cells. The response to ionising irradiation is represented by the linear-quadratic model. KD cells had significantly lower survival rate than UT cells (P < 0.05). TR-MRE11 cells had significantly lower survival rate than FL-MRE11 (P < 0.05) at 4 Gy. D The representative image of (C). E Flow cytometric analysis results of various RT112 cells after 8 Gy IR (48 h). F A statistical plot of annexin V-FITC/PI staining for (E) is shown for both early and late apoptosis after 4 and 8 Gy IR (48 h). The results are expressed as the means ± SD (****<0.0001, **<0.01, *P < 0.05).

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