Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 26;11:620843. doi: 10.3389/fmicb.2020.620843

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Nicholson, Waack, Anderson, Bayles, Zaia, Goertz, Eppinger, Hau, Brockmeier and Shore.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Comparison of phenotypes related to capsule production. (A) Bacterial hydrophobicity assay. Relative hydrophobicity of S. suis isolates were determined by measuring their absorption (y-axis) to n-hexadecane. S. suis P1/7 Δcps2E, a capsule deletion mutant, was used as a control. (B) Whole blood sensitivity assay. S. suis isolates were incubated with swine whole blood for 1 h and enumerated to determine the percent viable bacteria (y-axis) expressed. (C) Serum sensitivity assay. Bacteria were incubated with guinea pig serum (+Serum) or heat-inactivated guinea pig serum (HI + Serum) and enumerated to determine the percent survival (y-axis) calculated as the proportion of treated samples to untreated samples. Serum-sensitive Glasserella parasuis H465 (Hp H465) was used as a control. Bars represent means ± SEM from three independent experiments. Bar color reflective of virulence categorization (green: highly virulent; blue: moderately virulent; light gray: non-virulent) or control (dark gray: S. suis P1/7 Δcps2E). Data was analyzed using a one-way ANOVA with a Tukey’s post-test (GraphPad Prism 8.3.0). Groups with different letter designations were significantly different (p < 0.05); ns, not statistically significantly different.