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. 2021 Feb 8;11:116. doi: 10.1038/s41398-021-01229-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Experimental design for neurogenesis staining. b Representative images of EdU/DCX immunostaining in the dentate gyrus in mice of each group. Scale bar: 50 μm. c Higher-magnification images of the area marked by the frame in (b). Scale bar: 20 μm. d Quantification of EdU+ cells generated during the eight-day modafinil (MOD) treatment [F(2,6) = 14.2, p = 0.0053]. n = 3 per group. **p < 0.01 compared with the Sham+Vehicle (Veh) group; ^#p < 0.05 compared with ovariectomy (OVX)+Veh group. EdU 5-ethynyl-2′-deoxyuridine, DCX doublecortin.