Skip to main content
. 2021 Feb 8;11:3278. doi: 10.1038/s41598-021-82800-5

Table 3.

Stability values and rankings of ChP from the Development and the Light/Dark rearing panels calculated by four algorithms: geNorm, NormFinder, BestKeeper, DeltaCq and the overall ranking. Gene’s stability decreases from 1st to 12th rank.

Gene Overall geNorm NormFinder BestKeeper DeltaCq
Geometric mean of rank (GMR) Rank Stability value (M) Rank Stability value (ρ) Rank Stability value (r) Rank Mean SD of mean ΔCq Rank
Developmental panel
Rer1 2.783 1 0.216 5 0.207 2 0.922 3 0.40 2
Rpl13a 2.783  = 1 0.104 2 0.309 6 0.965 1 0.45 5
Gapdh 2.817 2 0.312 7 0.161 1 0.662 9 0.39 1
Rpl27 3.600 3 0.098 1 0.314 7 0.900 4 0.45 6
Tbp 4.949 4 0.156 4 0.438 10 0.878 5 0.43 3
B2m 5.009 5 0.376 9 0.278 5 0.953 2 0.45 7
Atp5f1 6.126 6 0.349 8 0.261 4 0.651 11 0.44 4
Actb 6.620 7 0.115 3 0.351 8 0.656 10 0.47 8
Ubc 6.817 8 0.258 6 0.248 3 0.472 12 0.54 10
Pgk1 8.972 9 0.410 10 0.363 9 0.781 8 0.52 9
Sdha 9.453 10 0.452 11 0.469 11 0.878 6 0.60 11
Hprt1 10.487 11 0.480 12 0.473 12 0.816 7 0.62 12
Light/Dark rearing panel
Hprt1 1.189 1 0.115 1 0.121 1 0.961 2 0.24 1
Rpl27 2.213 2 0.121 3 0.135 4 0.962 1 0.25 2
Tbp 2.711 3 0.205 6 0.132 3 0.949 4 0.26 3
Rpl13a 3.663 4 0.116 2 0.157 6 0.953 3 0.28 5
Atp5f1 4.527 5 0.220 7 0.123 2 0.940 5 0.29 6
Gapdh 5.318 6 0.182 5 0.151 5 0.962  = 1 0.31 8
Ubc 6.260 7 0.231 8 0.164 8 0.939 6 0.27 4
Rer1 6.817 8 0.135 4 0.189 10 0.939  = 6 0.32 9
Pgk1 7.454 9 0.240 9 0.163 7 0.917 7 0.30 7
Actb 9.212 10 0.253 10 0.166 9 0.899 8 0.33 10
Sdha 10.462 11 0.264 11 0.190 11 0.874 9 0.34 11
B2m 11.465 12 0.279 12 0.212 12 0.818 10 0.35 12