Table 3.
Stability values and rankings of ChP from the Development and the Light/Dark rearing panels calculated by four algorithms: geNorm, NormFinder, BestKeeper, DeltaCq and the overall ranking. Gene’s stability decreases from 1st to 12th rank.
| Gene | Overall | geNorm | NormFinder | BestKeeper | DeltaCq | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Geometric mean of rank (GMR) | Rank | Stability value (M) | Rank | Stability value (ρ) | Rank | Stability value (r) | Rank | Mean SD of mean Cq | Rank | |
| Developmental panel | ||||||||||
| Rer1 | 2.783 | 1 | 0.216 | 5 | 0.207 | 2 | 0.922 | 3 | 0.40 | 2 |
| Rpl13a | 2.783 | = 1 | 0.104 | 2 | 0.309 | 6 | 0.965 | 1 | 0.45 | 5 |
| Gapdh | 2.817 | 2 | 0.312 | 7 | 0.161 | 1 | 0.662 | 9 | 0.39 | 1 |
| Rpl27 | 3.600 | 3 | 0.098 | 1 | 0.314 | 7 | 0.900 | 4 | 0.45 | 6 |
| Tbp | 4.949 | 4 | 0.156 | 4 | 0.438 | 10 | 0.878 | 5 | 0.43 | 3 |
| B2m | 5.009 | 5 | 0.376 | 9 | 0.278 | 5 | 0.953 | 2 | 0.45 | 7 |
| Atp5f1 | 6.126 | 6 | 0.349 | 8 | 0.261 | 4 | 0.651 | 11 | 0.44 | 4 |
| Actb | 6.620 | 7 | 0.115 | 3 | 0.351 | 8 | 0.656 | 10 | 0.47 | 8 |
| Ubc | 6.817 | 8 | 0.258 | 6 | 0.248 | 3 | 0.472 | 12 | 0.54 | 10 |
| Pgk1 | 8.972 | 9 | 0.410 | 10 | 0.363 | 9 | 0.781 | 8 | 0.52 | 9 |
| Sdha | 9.453 | 10 | 0.452 | 11 | 0.469 | 11 | 0.878 | 6 | 0.60 | 11 |
| Hprt1 | 10.487 | 11 | 0.480 | 12 | 0.473 | 12 | 0.816 | 7 | 0.62 | 12 |
| Light/Dark rearing panel | ||||||||||
| Hprt1 | 1.189 | 1 | 0.115 | 1 | 0.121 | 1 | 0.961 | 2 | 0.24 | 1 |
| Rpl27 | 2.213 | 2 | 0.121 | 3 | 0.135 | 4 | 0.962 | 1 | 0.25 | 2 |
| Tbp | 2.711 | 3 | 0.205 | 6 | 0.132 | 3 | 0.949 | 4 | 0.26 | 3 |
| Rpl13a | 3.663 | 4 | 0.116 | 2 | 0.157 | 6 | 0.953 | 3 | 0.28 | 5 |
| Atp5f1 | 4.527 | 5 | 0.220 | 7 | 0.123 | 2 | 0.940 | 5 | 0.29 | 6 |
| Gapdh | 5.318 | 6 | 0.182 | 5 | 0.151 | 5 | 0.962 | = 1 | 0.31 | 8 |
| Ubc | 6.260 | 7 | 0.231 | 8 | 0.164 | 8 | 0.939 | 6 | 0.27 | 4 |
| Rer1 | 6.817 | 8 | 0.135 | 4 | 0.189 | 10 | 0.939 | = 6 | 0.32 | 9 |
| Pgk1 | 7.454 | 9 | 0.240 | 9 | 0.163 | 7 | 0.917 | 7 | 0.30 | 7 |
| Actb | 9.212 | 10 | 0.253 | 10 | 0.166 | 9 | 0.899 | 8 | 0.33 | 10 |
| Sdha | 10.462 | 11 | 0.264 | 11 | 0.190 | 11 | 0.874 | 9 | 0.34 | 11 |
| B2m | 11.465 | 12 | 0.279 | 12 | 0.212 | 12 | 0.818 | 10 | 0.35 | 12 |