Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 8;11:3308. doi: 10.1038/s41598-020-80813-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Schematic of extracellular vesicle (EV) segmentation using a blob detection algorithm. First, a large Gaussian filter was applied and then subtracted from the original intensity image to adjust for nonuniformity in the background. Next, Laplacian of Gaussian filters of different sizes were applied to the image to highlight local maxima of varying sizes. A 3D local maxima filter was applied to a stack of images filtered at different levels to create a mask for EVs of different sizes and a threshold was applied to only segment maxima that exceed the background noise. Finally, this binary mask was multiplied by the original intensity image to display the intensity of each segmented EV. Scale bars represent 20 μm.