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. 2021 Feb 8;11:3308. doi: 10.1038/s41598-020-80813-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorescence lifetime values of extracellular vesicles (EVs) and their parent cells for three cell lines: (a,b) MDA-MB-231 human epithelial breast cancer, (c,d) J774A.1 mouse macrophage, and (e,f) U-87 MG human glioblastoma. Mean and standard deviation of fluorescence lifetime values are indicated in the bar charts for three different replicates of each cell line, with each replicate indicated by color (replicate 1: red, replicate 2: blue, replicate 3: purple), in (a,c,e). Each replicate consisted of 4 fields-of-view (FOVs) for cells and 16 FOVs for EVs. One example FOV of each cell line is given as well: (b) MDA-MB-231, (d) J774A.1, and (f) U-87 MG. **Indicates P < 0.01, *** indicates P < 0.001. First replicate is indicated in red, second in blue, and third in purple. Scale bars represent 20 μm. The total number of vesicles (n) for each experimental group pooled together is as follows: MDA-MB-231 n = 561, J774A.1 n = 968, U-87 MG n = 178.