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. 2021 Feb 8;12:874. doi: 10.1038/s41467-021-21144-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Time course changes and corresponding modeling curves for the SgrS, ptsG mRNA, and SgrS-ptsG complex in a wild type, c G184A mutant strain, e G184A–C195U mutant strain, g G215A mutant strain. Average copy numbers per cell are plotted against time. Rate constants obtained for these mutants are shown in Fig. 6 and in Supplementary Table 3. Weighted R²’s for modeling are also reported in Supplementary Table 4. Data are presented as mean values ± SEM for n = 98, 90, 169, 144, 149, 127, 94, and 82 cells examined over two independent experiments after 0, 2, 4, 6, 8, 10, 15, and 20 min induction respectively for a; for n = 84, 84, 94, 99, 94, 90, 87, and 88 cells examined over two independent experiments after 0, 2, 4, 6, 8, 10, 15, and 20 min induction, respectively, for c; for n = 117, 101, 115, 111, 102, 110, 104, and 104 cells examined over two independent experiments after 0, 2, 4, 6, 8, 10, 15, and 20 min induction, respectively, for e and for n = 82, 88, 94, 104, 94, 90, 89, and 88 cells examined over two independent experiments after 0, 2, 4, 6, 8, 10, 15, and 20 min induction, respectively, for g. Histograms showing the change in distribution of SgrS and ptsG mRNA copy numbers for b wild type for n = 98, 90, 169, 144, 149, 127, 94, and 82 cells examined over two independent experiments after 0, 2, 4, 6, 8, 10, 15, and 20 min induction, respectively, d G184A mutant strain for n = 84, 84, 94, 99, 94, 90, 87, and 88 cells examined over two independent experiments after 0, 2, 4, 6, 8, 10, 15, and 20 min induction, respectively, f G184A–C195U mutant strain for n = 117, 101, 115, 111, 102, 110, 104, and 104 cells examined over two independent experiments after 0, 2, 4, 6, 8, 10, 15, and 20 min induction, respectively, h G215A mutant strain for n = 82, 88, 94, 104, 94, 90, 89, and 88 cells examined over two independent experiments after 0, 2, 4, 6, 8, 10, 15, and 20 min induction, respectively. Source data are provided as a Source data file.