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. 2021 Feb 8;11:3326. doi: 10.1038/s41598-021-82451-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparative analyses of pentapartite oral fluid (OF) fractions between healthy volunteers and patients with OSCC. (a) Western blot analyses of pentapartite fractions from six healthy subjects (HC7 to HC12) and six patients with OSCC (OSCC1 to OSCC6). Membranes were probed with antibodies against CD81, CD63, CD9, Alix, Hsp70, β-actin, AQP5, ACTN4, CD133, EGFR, and E-cadherin. Numbers on the right indicate molecular weight (MW) × 10^–3 of for MW markers. Black arrows show the reported sizes of the proteins. Nanoparticle tracking analyses (NTA) of particles contained in the 10K, 160K, and Sup fractions derived from healthy controls and patients with OSCC. Particle concentrations (b), and mode diameters (c), are indicated by green and red dots, respectively. The mean value of each group is represented as a solid horizontal line. The two-tailed unpaired t-test was used to evaluate statistical significance. Statistical significance was defined as p < 0.05. n.s. not significant.