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. 2021 Jan 26;11:618544. doi: 10.3389/fimmu.2020.618544

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Copyright © 2021 Haas, Rudolph, Costa, Faller, Libicher, Würthwein, Jarius, Ishikawa, Stump-Guthier, Tenenbaum, Schwerk, Schroten and Wildemann

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inverted transwell culture system. Inverted culture of HIBCPP cells on the bottom side of a transwell filter of 5 µm pore size. The upper (basolateral) side represents the lumen of the blood capillary, while the lower (apical) side mimics the CSF space. B cells are added to the upper compartment (blood side) and a cocktail of B-cell chemoattractants to the lower compartment. After 4 h transmigration (TM), filters are transferred onto a fresh plate; migrated cells are collected from the lower chamber and quantified by FACS. A chemotactic index (CI) is calculated for each B-cell subset.