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. 2021 Jan 26;11:618544. doi: 10.3389/fimmu.2020.618544

Figure 2.

Figure 2

Chemokine-induced transmigration of normal B cells across the HIBCPP monolayer. (A) TM rates of human total CD19+ B cells obtained from 15 healthy donors in the absence and presence of B-cell stimuli and chemokines. When various B-cell-specific chemokines were added alone or in combination to the lower chamber, TM rates multiplied by approximately 50-fold, with CXCL12 and CXCL13 being most effective. TM rates were further enhanced (approximately 1.5-fold) when B cells were activated with anti-CD40 & IgM during chemokine-triggered migration. All experiments were carried out in triplicates. Dots represent data per individuals. Means are indicated (grey circles). Error bars denote SD. Asterices indicate statistical significances compared with spontaneous TM (***P < 0.001, **P < 0.01; one-way ANOVA plus post Hoc Tukey test). Chemokine concentrations: CCL19 [500 ng/ml], CCL20 [100 ng/ml], CCL21 [120 ng/ml], CXCL12 [100 ng/ml], and CXCL13 [1 µg/ml]. “Mix” = all chemokines; “activated” = CD40 mAb [5 µg/ml] & IgM [12.5 µg/ml]. (B) Blocking of chemotactic activities by neutralizing mAbs. TM rates of CD40/IgM-stimulated B cells towards indicated chemokines in the presence of increasing concentrations of neutralizing mAbs. All experiments were carried out in triplicates.