Skip to main content
. 2021 Jan 26;11:618544. doi: 10.3389/fimmu.2020.618544

Figure 4.

Figure 4

Transmigration of MS-derived B-cell subsets across HIBCPP cells. Chemotactic activities of CD19+ total B cells, CD19+CD27+IgD- class-switched memory B cells (CSM), and CD19+CD27-IgD+ naive B cells isolated from peripheral blood of 15 healthy donors (HC) and 28 MS patients (untreated, in relapse: n = 10 (Rel); untreated, in remission: n = 8 (Rem); fingolimod-treated: n = 5 (FIN); natalizumab-treated: n = 5 (NAT)). (A) TM rates of total CD19+ B cells derived from treatment-naïve MS patients were not altered compared with controls, but where significantly lower when obtained from NAT-treated patients. (B) Overall, chemotactic indices (CI) of CSM B cells were clearly higher than those of CD27- naïve B cells. CIs where further enhanced for CSM B cells derived from non-treated MS but were significantly lower when tested with CSM B cells obtained from NAT-treated individuals. All experiments were carried out in triplicates. Dots represent data per individuals. Means are indicated (grey circles). Error bars denote SD. Asterices indicate statistical significances compared with spontaneous TM (*P < 0.05, n.s., not significant; one-way ANOVA plus post Hoc Tukey test).