Figure 4.

The proportion of CD3⁺ T cells, CD3⁺CD4^-CD8^- T cells, CD19⁺ B cells, CD19^-CD138⁺ cells (Plasma cells), CD3⁺S1PR1⁺ T cells and CD3⁺CD4⁺S1PR1⁺ T cells in spleen and early apoptotic cells in CD4⁺ and CD8⁺ T cells in lymph nodes were significantly increased in B6-Mir223^−/−Fas^lpr/lpr mice. (A) Various cellular subsets in lymph nodes and spleen. CD3⁺CD4⁺ (CD4 T cells), CD3⁺CD8⁺ (CD8 T cells), CD3⁺ CD4⁻CD8⁻ (Double negative (DN) T cells), CD19⁺ (B cells), CD19⁻CD138⁺ (Plasma cells), CD19⁺CD138⁺ (Plasmablasts), CD4⁺CD44⁺CD62L⁺ (Memory T cells), CD4⁺CD44⁺CD62L⁻ (Effector T cells), CD69⁺CD4⁺ cells, S1PR1⁺CD3⁺ cells, S1PR1⁺CD4⁺ cells. Distribution of subsets in total cells isolated from whole cervical lymph nodes and spleen were indicated in B6-Mir223^−/−Fas^lpr/lpr (n=7) and B6-Mir223^+/+Fas^lpr/lpr (n = 8) mice. Absolute number of total splenocyte and lymph nodes cell had no difference between two genotypes (Supplementary Figure 2E). (B) Apoptotic cells in lymph nodes and spleen. Annexin V⁺7-AAD⁻ (Early apoptosis cells), Annexin V⁺7-AAD⁺ (Late apoptotic cells). Distribution of subsets in CD3⁺CD4⁺ T cells or CD3⁺CD8⁺ T cells isolated from whole cervical lymph nodes and spleen were indicated in B6-Mir223^−/−Fas^lpr/lpr (n = 3) and B6-Mir223^+/+Fas^lpr/lpr (n = 3) mice. *p < 0.05, **p < 0.01, ns, not significant, by Student’s t-test in (A), by Wilcoxon signed-rank test in (B).