Fig. 2.

VSG2 transgenes were expressed and PTCs were effective in terminating translation.

A. Measurement of cell number over a five day time course for three independent clones of each transgenic cell line. Proliferation is expressed as cumulative cell number/10,000 and the standard error is shown (Supp. Table 2). Two panels are used for clarity.

B. Western blot of whole cell lysates for the series of cell lines expressing a VSG2 transgene in a VSG6 background. One clone of each cell line is shown: VSG6, the parental cell line expressing VSG6. VSG2, a distinct cell line expressing VSG2 from its endogenous locus. The remaining cell lines express a VSG2 transgene in a VSG6 background: VSG6 VSG2 WT, or WT VSG6 with PTCs at one of the following codons within VSG2: 64, 167, 262, 392, 426 or 460 were loaded on the gel. Whole cell lysates of 2 × 10⁶ cell equivalents were loaded per lane and the white asterix (*) indicates truncated VSG2 polypeptides of the expected predicted molecular weights.