Figure 2.

Effect of carbon deposition during HIM imaging. (a₁) HIM image (FOV 20 µm) of a cell infected at MOI 1 with charge compensation. (a₂) HIM images at high magnification (FOV 4.5 µm and 1 µm) with charge compensation. (a₃) The same image section as (a₁) after imaging the regions in (a₂). Due to increased conductivity, this region appears significantly brighter than the rest of the image. (b_1–3) HIM images of a cell infected at MOI 1 at different magnifications (FOV 20 µm, 5 µm, and 450 nm) with charge compensation. (c_1–3) HIM images of the same cell (FOV 20 µm, 2 µm, and 450 nm) after imaging the magnified sections in (b). (c₁) and (c₂) were imaged with and (c₃) was imaged without charge compensation.