Skip to main content
. 2021 Feb 2;49(2):0300060520986355. doi: 10.1177/0300060520986355

Figure 2.

Figure 2.

SIRT5 was detected in GC cell lines. SIRT5 overexpression promoted autophagy in HCG27 cells treated with 100 nM rapamycin. (a, c) Western blotting and quantification of SIRT5 expression in GC cell lines (MKN7, AGS, SUN1, and HCG27). (b, d) Western blotting and optical density quantification of SIRT5 levels in HCG27 cells after transfection with an SIRT5 lentivirus or control lentivirus. (e) Western blotting of SIRT5 expression and the LC3-II/LC3-I ratio in SIRT5-OE or vector cells incubated with or without rapamycin. (f) Relative optical density quantification of the LC3-II/LC3-I ratio. Data represent the average and SD of three or six independent experiments (*P < 0.05).

SIRT5, sirtuin 5; GC, gastric cancer; LC3-II, autophagosome-associated form of microtubule-associated protein 1 light chain 3; LC3-I, cytosolic form of microtubule-associated protein 1 light chain 3; SIRT5-OE, sirtuin 5-overexpressing lentivirus; Vector, control lentivirus.

HHS Vulnerability Disclosure