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. 2021 Feb 2;49(2):0300060520986355. doi: 10.1177/0300060520986355

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© The Author(s) 2021

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Autophagy is induced by rapamycin in HCG27 cells. (a) Immunofluorescent images of HCG27 cells after transfection with an SIRT5 lentivirus or control lentivirus and incubation with or without rapamycin. (b) Quantification of autophagy in HCG27 cells after transfection with an SIRT5 lentivirus or control lentivirus and incubation with or without rapamycin. Data represent the average and SD of three independent experiments (*P < 0.05).

SIRT5, sirtuin 5; SIRT5-OE, sirtuin 5-overexpressing lentivirus; Vector, control lentivirus.