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Rapamycin induced the formation of autophagic vacuoles in HCG27 cells. (a) Electron micrographs of autophagy in vector-transfected or SIRT5-OE HCG27 cells with or without 100 nM rapamycin. (b) The percentage of HCG27 cells with autophagosomes. (c) The average number of autophagic vacuoles per cell. Data represent the average and SD of three independent experiments (*P < 0.05).
SIRT5, sirtuin 5; SIRT5-OE, sirtuin 5-overexpressing lentivirus; Vector, control lentivirus.
