Table 1.
The primer pairs of lnc-ATG9B-4.
| Names | Nucleotide Sequence (5ʹ-3ʹ) | |
|---|---|---|
| lnc-ATG9B-4 PCR | Forward | ATCGGGATTCCAGGCAGATCACCCGAGG |
| Reverse | ATCGTCTAGAGACACTCCAGTCGCCAGC | |
| lnc-ATG9B-4 qPCR | Forward | ATTGGCTCTTTATCCCTGCTGA |
| Reverse | AGTGGTGCTTCCAGGATTCAA | |
| 18s RNA | Forward | AGAAACGGCTACCACATCCA |
| Reverse | CACCAGACTTGCCCTCCA | |
| SiRNA-control | TTCTCCGAACGTGTCACGTAA | |
| shRNA-control | Top strand | GATCCGTTCTCCGAACGTGTCACGTAATTCAAGAGATTACGTGACACGTTCGGAGAATTTTTTC |
| Bottom strand | AATTGAAAAAATTCTCCGAACGTGTCACGTAATCTCTTGAATTACGTGACACGTTCGGAGAACG | |
| siRNA1 | GAGAATTGCTTGAACCCAGGAGGCAGAGAATTGCTTGAACCCAGGAGGCA | |
| shRNA1-lnc-ATG9B-4 | Top strand | GATCCGAGAATTGCTTGAACCCAGGAGGCATTCAAGAGATGCCTCCTGGGTTCAAGCAATTCTCTTTTTTG |
| Bottom strand | AATTCAAAAAAGAGAATTGCTTGAACCCAGGAGGCATCTCTTGAATGCCTCCTGGGTTCAAGCAATTCTCG | |
| siRNA2 | GGCCTATTAGATTGGCTCTTTATCC | |
| shRNA2-lnc-ATG9B-4 | Top strand | GATCCGGCCTATTAGATTGGCTCTTTATCCTTCAAGAGAGGATAAAGAGCCAATCTAATAGGCCTTTTTTG |
| Bottom strand | AATTCAAAAAAGGCCTATTAGATTGGCTCTTTATCCTCTCTTGAAGGATAAAGAGCCAATCTAATAGGCCG | |
| siRNA3 | TCCAAAGTGCTGGGATTACAGGAGC | |
| shRNA3-lnc-ATG9B-4 | Top strand | GATCCGTCCAAAGTGCTGGGATTACAGGAGCTTCAAGAGAGCTCCTGTAATCCCAGCACTTTGGATTTTTTG |
| Bottom strand | AATTCAAAAAATCCAAAGTGCTGGGATTACAGGAGCTCTCTTGAAGCTCCTGTAATCCCAGCACTTTGGACG | |