Figure 4.

CCAT1 directly binds to miR-216a-5p and suppresses its expression. (a) The potential binding sites between CCAT1 and miR-216a-5p. (b and c) Luciferase reporter assay was used to demonstrate whether CCAT1 directly targeted miR-216a-5p in A549 and NCI-H1299 cells, respectively. (d) RIPA was carried out, and expression of CCAT1 and miR-216a-5p was examined in the samples bound to the Ago2 or IgG antibody. (e) CCAT1 expression level was measured by qRT-PCR in samples pulled down by Bio-miR-216a-5p or negative control. (f) qRT-PCR analysis of miR-216-5p level in A549 and NCI-H1299 cells transfected with CCAT1 or si-CCAT1 was conducted. *P < 0.05. (A color version of this figure is available in the online journal.)