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. 2020 Oct 6;246(2):142–152. doi: 10.1177/1535370220961013

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Figure 6. — MiR-216a-5p directly targets RAP2B and negatively regulates RAP2B expression. (a) The potential binding sites between RAP2B and miR-216a-5p. (b) Luciferase reporter assay was used to analyze whether miR-216a-5p directly targeted RAP2B in A549 and NCI-H1299 cells, respectively. (c and d) The expression of RAP2B was detected in A549 and NCI-H1299 cells after RIPA. (e) The protein expression of RAP2B was analyzed using Western blot in A549 and NCI-H1299 cells transfected with miR-216a-5p or anti-miR-216a-5p. *P < 0.05. (A color version of this figure is available in the online journal.)