Figure 7.

Overexpressed miR-216a-5p inhibits NSCLC progression through targeting RAP2B. RAP2B expression in NSCLC tissues and matched adjacent normal lung tissue (a), as well as in human NSCLC cell lines (A549 and NCI-H1299) and BEAS-2B cell lines (b) was detected by qRT-PCR. RAP2B expression detection by Western blot (c), cell proliferation by CCK-8 assay (d), cell apoptosis by flow cytometry (e), levels of apoptosis-related proteins analysis by Western blot (f), cell migration and invasion abilities by transwell assay (g and h) in A549 and NCI-H1299 cells transfected with NC, miR-216a-5p, miR-216a-5p + vector, or miR-216a-5p + RAP2B. *P < 0.05. (A color version of this figure is available in the online journal.)