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. 2021 Feb 4;14:297–310. doi: 10.2147/JPR.S291472

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© 2021 Dong et al.

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The protective effect of exosomal miR-127-3p on chondrocytes. (A) Prediction on the miRNA (Log2 expression) that is enriched in MSC-derived exosomes. (B) The effect of extracted exosomes on the expression of related miRNAs in chondrocytes by RT-qPCR. (C) RT-qPCR utilized to detect miR-127-3p expression in the removed supernatant during the exosome extraction and in the exosomes. (D) The transfection efficiency of miR-127-3p inhibitor in BM-MSCs confirmed by RT-qPCR. (E) miR-127-3p expression in the extracted exosomes (Exo-NC and Exo-inhibitor) determined by RT-qPCR. (F) Effects of Exo-NC and Exo-inhibitor treatment on the viability of chondrocytes measured by MTT assay. (G) Effects of Exo-NC and Exo-inhibitor treatment on the DNA synthesis activity of chondrocytes measured by EdU assay. (H) Effects of Exo-NC and Exo-inhibitor treatment on the apoptosis rate of chondrocytes determined by flow cytometry. (I) Effects of Exo-NC and Exo-inhibitor treatment on the expression of Collagen II and MMP13 in chondrocytes determined by Western blot assay. All the experiments were performed independently in triplicate and the results were the mean of three values. Unpaired t test (C–H) was utilized for comparison between two groups or two-way ANOVA (B, I) was used for comparison among multiple groups. *p < 0.05 vs OA group; ###p < 0.05 vs Supernatant group; @p < 0.05 vs NC inhibitor group; &p < 0.05 vs Exo-NC group.

Abbreviations: miRNA, microRNA; MTT, 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium; EdU, 5-ethynyl-2′-deoxyuridine; RT-qPCR, reverse transcription quantitative polymerase chain reaction; Exo, exosome; NC, negative control; MMP13, matrix metalloprotease 13; ANOVA, analysis of variance.