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. 2021 Feb 4;14:297–310. doi: 10.2147/JPR.S291472

Figure 4.

Figure 4

CDH11 is a downstream target gene of miR-127-3p. (A) The potential target genes of miR-127-3p in rats predicted in miRDB (http://www.mirdb.org/), miRWalk (http://mirwalk.umm.uni-heidelberg.de/), and TargetScan (http://www.targetscan.org/vert_71/). (B) RT-qPCR detection of the transfection efficiency of miR-127-3p mimic in chondrocytes. (C) Effect of miR-127-3p mimic on mRNA expression of potential target genes by RT-qPCR. (D) Effect of miR-127-3p mimic on CDH11 protein expression determined by Western blot assay. (E) Potential binding sites of miR-127-3p and CDH11. (F) Effect of miR-127-3p on CDH11-WT/CDH11-MT luciferase activity confirmed by luciferase activity assay. (G) RIP assay utilized to detect the binding relationship between miR-127-3p and CDH11. (H) Effects of miR-127-3p mimic and pcDNA-CDH11 on the expression of CDH11 and OA-related genes MMP13, IL-6, TNF-α, and ADAMTS-5 in chondrocytes examined by RT-qPCR. All the experiments were performed independently in triplicate and the results were the mean of three values. Unpaired t test (B, D) was utilized for comparison between two groups or two-way ANOVA (C–H) was used for comparison among multiple groups. *p < 0.05 vs NC mimic group; ##p < 0.05 vs Anti-IgG group; &p < 0.05 vs miR-127-3p mimic + pcDNA group.

Abbreviations: miRNA, microRNA; RT-qPCR, reverse transcription quantitative polymerase chain reaction; CDH11, Cadherin-11; WT, wild type; MT, mutant; MMP13, matrix metalloprotease 13; IL-6, interleukin-6; TNF-α, tumor necrosis factor-α; ADAMTS-5, a disintegrin and metalloproteinase with thrombospondin type 1 motifs-5.