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. 2021 Feb 4;14:297–310. doi: 10.2147/JPR.S291472

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© 2021 Dong et al.

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Exosomes carrying miR-127-3p inhibit CDH11 expression in chondrocytes. (A) Effects of IL-1β and exosome treatment on the expression of CDH11 in chondrocytes detected by RT-qPCR. (B) RT-qPCR for detecting the transfection efficiency of pcDNA-CDH11. (C) Effects of upregulated CDH11 on the viability of chondrocytes measured by MTT assay. (D) Effects of upregulated CDH11 on the DNA synthesis activity of chondrocytes measured by EdU assay. (E) Effects of upregulated CDH11 on the apoptosis rate of chondrocytes determined by flow cytometry. (F) Effects of upregulated CDH11 on the expression of Collagen II and MMP13 in chondrocytes determined by Western blot assay. All the experiments were performed independently in triplicate and the results were the mean of three values. Unpaired t test (B–E) was utilized for comparison between two groups or one-way ANOVA (A)/two-way ANOVA (F) was used for comparison among multiple groups. *p < 0.05 vs control group; #p < 0.05 vs OA group; &p < 0.05 vs Exo + pcDNA group.

Abbreviations: miRNA, microRNA; RT-qPCR, reverse transcription quantitative polymerase chain reaction; CDH11, Cadherin-11; MTT, 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium; EdU, 5-ethynyl-2′-deoxyuridine; Exo, exosome; NC, negative control; MMP13, matrix metalloprotease 13; ANOVA, analysis of variance; OA, osteoarthritis.