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. 2021 Feb 4;14:297–310. doi: 10.2147/JPR.S291472

Table 1.

Sequence Used for RT-qPCR

Targets Forward Primer (5ʹ-3ʹ) Reverse Primer (5ʹ-3ʹ)
miR-127-3p CTGAAGCTCAGAGGGCTCTG GAACATGTCTGCGTATCTC
CDH11 ACACAGGATGGTGTGGTGAAGC CTTGACGGTCACAGTGTCCTTG
U6 CTCGCTTCGGCAGCACAT TTTGCGTGTCATCCTTGCG
GAPDH CATCACTGCCACCCAGAAGACTG ATGCCAGTGAGCTTCCCGTTCAG
miR-4792 CGGTGAGCGCTCGCTGG GAACATGTCTGCGTATCTC
miR-6089 GGAGGCCGGGGTGGGGCGGGG GAACATGTCTGCGTATCTC
miR-1246 AATGGATTTTTGGAGCAGG GAACATGTCTGCGTATCTC
miR-4466 GGGTGCGGGCCGGCGG GAACATGTCTGCGTATCTC
miR-7641 TTGATCTCGGAAGCTAAGC GAACATGTCTGCGTATCTC
miR-3665 AGCAGGTGCGGGGCGG GAACATGTCTGCGTATCTC
miR-658 AGGGAAGTAGGTCCGTT GAACATGTCTGCGTATCTC
miR-1273 GAACCCATGAGGTTGAGGCT GAACATGTCTGCGTATCTC
miR-7704 CGGGGTCGGCGGCGACG GAACATGTCTGCGTATCTC
KIF3B CGAACAGCAGAAGATCCTGGAG TCAGTTCCAGGGTCTCCTCATC
ING5 AGATCCAGAGCGCCTACAGCAA CAGGTCAGCATCAAGTCTTCGG
ATP2B2 CGGATAAGCACACGCTGGTCAA AGCCCACATCTGCCTTCTTGAG
MMP13 AACCAAGATGTGGAGTGCCTGATG CACATCAGACCAGACCTTGAAGGC
IL-6 GGTGCTAGGAGAAAACACCCA CCACTGGCATGGGGACTAAC
TNF-α TTTCTGTGGTACCCTCTGTGC GATCGGTCCCAACAAGGAGG
ADAMTS-5 TGTGGCTGAATAGCCCATCC TTCACGTGGACATCCCTGC

Abbreviations: miR, microRNA; GAPDH, glyceraldehyde phosphate dehydrogenase; RT-qPCR, reverse transcription quantitative polymerase chain reaction; CDH11, Cadherin-11; KIF3B, kinesin family member 3B; ING5, inhibitor of growth family 5; ATP2B2, ATPase, calcium-transporting, plasma membrane 2; MMP13, matrix metalloprotease 13; IL-6, interleukin-6; TNF-α, tumor necrosis factor-α; ADAMTS-5, a disintegrin and metalloproteinase with thrombospondin type 1 motifs-5.