Table 1.
Targets | Forward Primer (5ʹ-3ʹ) | Reverse Primer (5ʹ-3ʹ) |
---|---|---|
miR-127-3p | CTGAAGCTCAGAGGGCTCTG | GAACATGTCTGCGTATCTC |
CDH11 | ACACAGGATGGTGTGGTGAAGC | CTTGACGGTCACAGTGTCCTTG |
U6 | CTCGCTTCGGCAGCACAT | TTTGCGTGTCATCCTTGCG |
GAPDH | CATCACTGCCACCCAGAAGACTG | ATGCCAGTGAGCTTCCCGTTCAG |
miR-4792 | CGGTGAGCGCTCGCTGG | GAACATGTCTGCGTATCTC |
miR-6089 | GGAGGCCGGGGTGGGGCGGGG | GAACATGTCTGCGTATCTC |
miR-1246 | AATGGATTTTTGGAGCAGG | GAACATGTCTGCGTATCTC |
miR-4466 | GGGTGCGGGCCGGCGG | GAACATGTCTGCGTATCTC |
miR-7641 | TTGATCTCGGAAGCTAAGC | GAACATGTCTGCGTATCTC |
miR-3665 | AGCAGGTGCGGGGCGG | GAACATGTCTGCGTATCTC |
miR-658 | AGGGAAGTAGGTCCGTT | GAACATGTCTGCGTATCTC |
miR-1273 | GAACCCATGAGGTTGAGGCT | GAACATGTCTGCGTATCTC |
miR-7704 | CGGGGTCGGCGGCGACG | GAACATGTCTGCGTATCTC |
KIF3B | CGAACAGCAGAAGATCCTGGAG | TCAGTTCCAGGGTCTCCTCATC |
ING5 | AGATCCAGAGCGCCTACAGCAA | CAGGTCAGCATCAAGTCTTCGG |
ATP2B2 | CGGATAAGCACACGCTGGTCAA | AGCCCACATCTGCCTTCTTGAG |
MMP13 | AACCAAGATGTGGAGTGCCTGATG | CACATCAGACCAGACCTTGAAGGC |
IL-6 | GGTGCTAGGAGAAAACACCCA | CCACTGGCATGGGGACTAAC |
TNF-α | TTTCTGTGGTACCCTCTGTGC | GATCGGTCCCAACAAGGAGG |
ADAMTS-5 | TGTGGCTGAATAGCCCATCC | TTCACGTGGACATCCCTGC |
Abbreviations: miR, microRNA; GAPDH, glyceraldehyde phosphate dehydrogenase; RT-qPCR, reverse transcription quantitative polymerase chain reaction; CDH11, Cadherin-11; KIF3B, kinesin family member 3B; ING5, inhibitor of growth family 5; ATP2B2, ATPase, calcium-transporting, plasma membrane 2; MMP13, matrix metalloprotease 13; IL-6, interleukin-6; TNF-α, tumor necrosis factor-α; ADAMTS-5, a disintegrin and metalloproteinase with thrombospondin type 1 motifs-5.