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. Author manuscript; available in PMC: 2021 Feb 9.
Published in final edited form as: Nat Struct Mol Biol. 2015 Sep 21;22(10):809–814. doi: 10.1038/nsmb.3099

Figure 4.

Figure 4

Cysteine accessibility by malPEG labeling. (a) In vitro labeling schematic of AfCcdA mutants with 5-kDa malPEG. (b) SDS-PAGE of AfCcdA(CA) and (AC) malPEG labeling after 60 min (lanes 2 and 5) versus negative (lanes 1 and 4) and positive (lanes 3 and 6) controls. (c) 1H-15N TROSY-HSQC spectra of malPEG-labeled AfCcdA(AC) (purple) and AfCcdA(CA) (green). (d) Major W115 and minor W115* side chain indole peaks.