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EM images of the tubulin samples used for turbidimetry. EM images of WT and Y222F tubulin 1 min after the ultracentrifugation (top) and 30 min later (bottom) at 4°C. Although our ultracentrifugation condition (see Materials and methods) should sediment oligomers that are larger than dimers, we sometimes observed a tetramer in the Y222F sample immediately after ultracentrifugation, which may have been assembled during the 1 min needed for the preparation of the EM grid.
