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. 2021 Jan 29;20:1533033821990040. doi: 10.1177/1533033821990040

Figure 3.

Figure 3.

Circ_0003998 in DTX-resistant NSCLC cells directly interacted with miR-136-5p. The level of miR-136-5p by qRT-PCR in 25 primary NSCLC tissues (sensitive), 25 recurrent NSCLC tissues (resistant) and 30 matched healthy lung tissues (normal) (A), 16HBE, A549, H1299, A549/DTX and H1299/DTX cells (B). (C) Correlation between miR-136-5p expression and circ_0003998 level in 50 NSCLC tissues using Spearman test. (D) miR-136-5p expression by qRT-PCR in A549/DTX and H1299/DTX cells transfected with mimic NC or miR-136-5p mimic. (E) Schematic of the putative target sequence for miR-136-5p within circ_0003998 and the mutation of the seed region. (F and G) The levels of circ_0003998 and miR-136-5p by qRT-PCR in cell lysates incubated with anti-IgG or anti-Ago2 antibody. (H) Dual-luciferase assays in 293 T cells cotransfected with mimic NC or miR-136-5p mimic and circ_0003998 wt or circ_0003998 mut. *P < 0.05, **P < 0.01, ***P < 0.001 or ### P < 0.001.