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. 2021 Feb 9;22:109. doi: 10.1186/s12864-021-07416-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Comparative analysis of transcriptome profiles of linseed seedling leaves under DS and RD. a Principal component analysis (PCA) of mRNA populations from the control, DS, RW and RD groups. Each sample contained two replicates. Principal components (PCs) 1 and 2 account for 30 and 22% of the variance, respectively. The PCA plot shows four distinct groups of mRNA populations. Group I: Z141 CK (blue square) and NY-17 CK (red square); group II: Z141 DS (blue diamond) and NY-17 DS (red diamond); group III: Z141 RW (blue circle) and NY-17 RW (red circle) and group IV: Z141 RD (blue triangle) and NY-17 RD (red triangle). b Hierarchical clustering of DEGs exhibiting altered expression levels in response to CK, DS, RW and RD treatments. The colours in the scale (blue (low), white (medium) and red (high)) represent the normalized expression levels of differentially expressed DEGs. c, d Venn diagrams showing overlap of up- (c) or downregulated (d) genes in response to the four assayed abiotic stresses: Z141-DS (purple), NY-17-DS (yellow), Z141-RD (green) and NY-17-RD (red)