Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 8;20:39. doi: 10.1186/s12934-021-01529-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — SDS-PAGE and Western immunoblot on extracts of the Synechocystis strains created in this study. SDS-PAGE/Western immunoblot analysed for the presence of ICL and MS (a); analysed for the presence of PEPc (b). a Upper panel shows SDS-PAGE loaded with 5 µg of protein crude extract from the Synechocystis engineered strains created in this study (Table 1). Lower panel shows Western immunoblot using anti-Strep antibody for the Synechocystis engineered strains (Table1). Bands correspond to MS (approximate molecular weight 60 kDa) and to ICL (approximate molecular weight 47 kDa). Samples are from cells incubated under darkness. b Upper panel shows SDS-PAGE loaded with 31 µg of protein crude extract from Synechocystis strains created in this study (Table 1). Lower panel displays Western immunoblot using anti-PEPc for the Synechocystis engineered strains. The approximate molecular weight of PEPc is 110 kDa. Control (CTL) corresponds to purified PEPc from the cyanobacterium Synechocystis PCC 6803. Samples are from cells incubated under anoxic darkness