Table 2.
Plasmids and oligonucleotides used/created in this study
| Primer/ Plasmid |
Sequence/Construct |
|---|---|
| oligoF-AXS | TATGTCTAGAATGTGGAGTCATCCTCAGTTCGAGAAGGGTAGCGGAAGTAAAACCCGTACACAACAAAT |
| oligoR-ABP | TGAACTGCAGTAACTTAGATCTTTAGAACTGCGATTCTTCGG |
| oligoF-BBRS | CTTAAGATCTTAGTGGAGGTAAGCTTATGTGGAGTCATCCTCAGTTCGAGAAGGGTAGCGGAAGTACTGAACAGGCAACAACAACC |
| oligoR-BP | TGAACTGCAGTTACGCTAACAGGCGGTAGC |
| pEERM3 + | Plasmid (empty backbone) for integration into neutral site of Synechocystis 6803, based on the pJ344 vector backbone, carrying chloramphenicol resistance cassette, PnrsB promoter and the synthetic RBS, RBS* |
| pEERM3-aceA | As pEERM3, but with E. coli aceA expressed from the PnrsB promoter |
| pEERM3-aceAB | As pEERM3, but with E. coli aceAB expressed from the PnrsB promoter |