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. 2021 Feb 8;14:27. doi: 10.1186/s13041-021-00745-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Average current densities of Ca_V2.1 (Δ47), Y1384C (Δ47), Ca_V2.1 (+ 47) and Y1384C (+ 47) channels. a Representative whole cell Ca²⁺ current traces recorded in response to depolarizing steps from − 60 mV to + 30 mV from a holding potential of − 100 from tsA-cells expressing either Ca_V2.1(Δ47), Y1384C (Δ47), Ca_V2.1(+ 47) or Y1384C (+ 47) α1 subunit with Ca_Vβ₄ and Ca_Vα₂δ-1. b Average current density–voltage relationships for cells expressing Ca_V2.1(Δ47) and Y1384C (Δ47) channels. c Average current density–voltage relationships for cells expressing Ca_V2.1(+ 47) and Y1384C (+ 47) channels. d Average peak current density recorded from cells expressing Ca_V2.1 (Δ47), Y1384C (Δ47), Ca_V2.1 (+ 47) and Y1384C (+ 47) channels. e Left: Representative blot of Ca_V2.1 (Δ47) and Y1384C (Δ47) channels surface expression (top blot) and Na/K ATPase surface expression (bottom blot). Right: Quantification of plasma membrane Ca_V2.1 (Δ47) and Y1384C (Δ47) channel expression normalized by Na/K ATPase cell surface expression. Data are from 3 independent experiments. f Percentage of remaining current at the end of the 250 ms test pulse with respect to the peak current amplitude. The numbers in parentheses represent the numbers of cells recorded. Asterisks denote significance *0.05, **0.01, ***0.001 levels (One-way ANOVA, and Tukey’s multiple comparison test, with a single pooled variance test)