Fig. 3.

Potential capability of CD8⁺ T and CD4⁺ T cells in PBMCs of HD, SCR and LCR cohorts to secrete cytokines. A, B PBMCs were co-stained with phenotypic markers and nuclear transcription factor T-bet, representative plots and cumulative frequencies of T-bet⁺ CD8⁺ T (A) and CD4⁺ T (B) cells were shown. C–I PBMCs were stimulated with PMA and ionomycin for 4.5 h in the presence of BFA and monensin. Production of IFN-γ (C, D), IL-2 (E, F), granzyme B (G, H), IL-4 (I, J) and IL-17A (K, L) by CD8⁺ T or by CD4⁺ T cells were intracellularly stained and analyzed by flow cytometry. Results are shown as mean ± S.D. Data were analyzed with one-way ANOVA. ns, non-significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.