Skip to main content
. 2018 Dec 14;36(1):165–175. doi: 10.1089/neu.2017.5619

FIG. 2.

FIG. 2.

Cross-reactivity Kir6.2-Kir6.1. (A) Staining of Kir6.1 (green; APC-105; Alomone Labs) and Kir6.2 (red; APC-020; Alomone Labs) in the same section of mouse brain tissue. Kir6.1 shows labeling in blood vessel-like structures and Kir6.2 in neuronal-like structures. This shows that the Kir6.2 antibody does not cross-react with the Kir6.1 epitope. (B) Western blot analysis of a lysate of HEK293T-cells transfected with an empty vector (lane 1) and HEK293T cells that overexpress Kir6.1 (NBL1-12174; Novus Biological, Littleton, CO) (lane 2). The membrane shown on the left was incubated with an anti-Kir6.1 antibody and the one on the right with an anti-Kir6.2 antibody. Only the Kir6.1 antibody was detecting the HEK293T-cells lysate showing that cross-reactivity does not occur between the Kir6.2 antibody and the Kir6.1 epitope.