Table 3.

Primers used for constructing mutants

Name	Sequence (5ʹ → 3ʹ)
spl-UP-For	CCGAATTCCATAGTTAGAACAATGAATTAG
spl-UP-Rev	GGACCTCCGCGGCTCCTTTACAATTTGAGATA
spl-DOWN-For	GGACCTCCGCGGTAAGAAATTCATTGCAGATA
spl-DOWN-Rev	CCGTCGACTTTCAGATAAGAAGCAATAG
rot-UP-attB1-For	GGGGACAAGTTTGTACAAAAAAGCAGGCTGTATTAGGGGTAGGACTTGGCGGTTTG
rot-UP-Rev	ATGACTGGATCCCTACAAGTGTAAATAAACTTGCTTTC
sigB-UP-attB1-For	GGGGACAAGTTTGTACAAAAAAGCAGGCTCGGATGGTGTGACTGAAGCTAGAAATAGTG
sigB-UP-Rev	ATGACTGGATCCCCATTGGTTAATTTGCTCAGGTGAAACTTC
rot-DOWN-For	ATGACTGGATCCGATTGCAAGTAGAGCAACAGCAATGC
rot-DOWN-attB2-Rev	GGGGACCACTTTGTACAAGAAAGCTGGGTGAAGACTATGACATGATGATGGCTACTGCC
sigB-DOWN-For	ATGACTGGATCCCGGCAATTAAGAAATTACAAGAAGCAGCAC
sigB-DOWN-attB2-Rev	GGGGACCACTTTGTACAAGAAAGCTGGGTTTAGATGTAGTTAACACACCTTGATGATAC