Figure 4.

Distribution of avian influenza virus matrix protein (M1) in selected organs of chicken embryos. Distribution of influenza M1 protein in the indicated organs of embryos inoculated with different viruses. 14-day-old embryonated chicken eggs were inoculated via the allantoic sac for 5 days then embryos were subjected to immunohistochemistry using primary anti-matrix antibody for antigen detection. Labeling by 3-amino-9-ethyl-carbazol (red-brown); hematoxylin counterstain (blue). Bars = 50 µm