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. Author manuscript; available in PMC: 2021 Feb 19.
Published in final edited form as: Nature. 2020 Aug 19;586(7830):606–611. doi: 10.1038/s41586-020-2631-z

Extended Data Fig. 6. Immunofluorescence characterization of wHILOs.

Extended Data Fig. 6.

a-c, Confocal images of wHILOs stained for C-peptide (a), β cell enriched markers NKX2–2, NKX6–1, MAFA, MAFB, PDX1 (b), and endocrine markers chromogranin A (CHGA), Synaptophysin (red) with Insulin-GFP (green) visualization (c). (d) Magnification of 75μm x75μm boxed regions shown in (b) and (c). (e) Immunofluorescence images of wHILOs showing insulin (GFP), β cell markers MAFA and MAFB, and α cell marker glucagon expression. Hoechst nuclei staining (blue). Scale bar: 100 μm panels a-c, 10μm panel e. Images are representative of 3 independent experiments.