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. Author manuscript; available in PMC: 2021 Feb 19.
Published in final edited form as: Nature. 2020 Aug 19;586(7830):606–611. doi: 10.1038/s41586-020-2631-z

Fig. 2. Exogenous PD-L1 expression extends wHILO functionality in immune competent mice.

Fig. 2.

a, Representative immunofluorescence staining for glucagon, somatostatin and pancreatic polypeptide (PP) in wHILOs. b, tSNE clustering of combined wHILO (blue dots, n=4840) and human islet (red dots, n= 3245) single cell transcriptomes (left panel) and clustering analysis-defined cell types (left). c, Schematic of experimental program. High dose streptozotocin (HD-STZ, 180mg/kg) induced diabetic C57BL6J mice received transplants of wHILOs with and without PD-L1 overexpression (n=500), or mouse islets. d, Random fed blood glucose levels after transplantation of wHILOs with or without PD-L1 expression (n=11 and 9, respectively), and C57BL6J islets (n=7). e, Flow cytometric analysis of insulin-expressing and mouse immune (CD45+) cells recovered from kidney capsule grafts 27 days after transplantation of wHILOs with and without PD-L1 expression (n=6). f, Quantification of analyses in (d). Error bars represent ± SEM. *p<0.05, **p<0.01, ***p<0.001, one-tailed, student’s paired t test. Images are representative of 3 independent experiments. Data were representative (e), or compiled from 3 independent samples (b) or experiments (d, f).